Influence of hydrophilic polymers on the accelerated blood clearance of mRNA lipid nanoparticles upon repeated administration
Dayangku Nordiyana B P Hassanel, Yi Ju, Asuka Takanashi, Azizah Algarni, Chee Leng Lee, Stephen J. Kent, Colin W. Pouton, Emily H. Pilkington
Abstract
-(2-hydroxypropyl) methacrylamide) (PHPMA). Our results demonstrated that all three POEGMA LNPs, especially POEGMA (+) LNPs, exhibited minimal ABC effect after two weekly doses; in contrast, PDMA LNPs demonstrated significantly lower clearance in the presence of anti-PEG antibodies. This study highlights the potential of PEG-free polymer-LNPs as promising mRNA carriers that avoid rapid clearance with repeated administration.
Topics & Concepts
ChemistryAntibodyEtherClearance rateEthylene glycolMessenger RNAPEG ratioPharmacologyBiochemistryPolymerPoloxamerDrug carrierAntibody therapyClearanceMethacrylateNanoparticleIn vivoBlood substitutePEGylationRNA Interference and Gene DeliveryAdvanced Polymer Synthesis and CharacterizationBiopolymer Synthesis and Applications