Electrochemical Fabrication of Poly (niacin) Modified Graphite Paste Electrode and its Application for the Detection of Riboflavin
J. G. Manjunatha, C. Raril, N. Hareesha, M. M. Charithra, Pemmatte A. Pushpanjali, Girish Tigari, Doddarasinakere K. Ravishankar, S. C. Mallappaji, Jayarame Gowda
Abstract
Objective: A poly niacin (NN) modified graphite paste electrode was prepared through a simple electrochemical polymerization route for the determination of Riboflavin (RF) Methods: Electropolymerization technique was used for the preparation of a modified electrode. The electrochemical impedance spectroscopy (EIS) method was used to study the surface behaviors and conductivity of the bare and modified electrodes. Cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were used to study the electrochemical behavior of RF using 0.2 M PBS of 7.5 pH. Results: Poly niacin modified graphite paste electrode (PNNMGPE) provides more affinity towards RF detection with high sensitivity and selectivity over bare graphite paste electrode. RF calibration curve was obtained by LSV between 5.0 µM to 65.0 µM with 0.782 µM limit of detection (LOD) and 2.7608 µM limit of quantification (LOQ), respectively. Selective analysis of RF in the presence of ascorbic acid (AA) and dopamine (DA) was performed at PNNMGPE by the LSV method. Conclusion: The developed PNNMGPE was tested for RF analysis in a multivitamin tablet with good results. Furthermore, the developed electrode shows good stability and reproducibility.