Surface Passivation with a Perfluoroalkane Brush Improves the Precision of Single-Molecule Measurements
Carlos J. Bueno-Alejo, Marina Santana Vega, Amanda K. Chaplin, Chloe Farrow, Alexander Axer, Glenn A. Burley, Cyril Dominguez, Hesna Kara, Vasileios Paschalis, Sumera Tubasum, Ian C. Eperon, Alasdair W. Clark, Andrew J. Hudson
Abstract
single-molecule experiments using iSCAT microscopy because it allows for continuous monitoring of adsorption and desorption of protein without either a decline in detection events or a change in sample composition due to the irreversible binding of protein to surfaces.
Topics & Concepts
AdsorptionDesorptionPassivationMoleculeMaterials scienceAqueous solutionMonomerProtein adsorptionChemical engineeringChemical physicsAnalytical Chemistry (journal)NanotechnologyChemistryPhysical chemistryOrganic chemistryPolymerLayer (electronics)EngineeringComposite materialNanofabrication and Lithography TechniquesAdvanced Biosensing Techniques and ApplicationsPolymer Surface Interaction Studies