Gelatin and catechol-modified quaternary chitosan cotton dressings with rapid hemostasis and high-efficiency antimicrobial capacity to manage severe bleeding wounds
Lin Li, Dongfan Chen, Jiale Chen, Chao Yang, Youyun Zeng, Ting Jin, Yanmei Zhang, Xiaoyu Sun, Hongli Mao, Zhixiang Mu, Xinkun Shen, Zhanwei Ruan, Xiaojun Cai
Abstract
The development of functional wound dressings capable of both rapid hemostasis and high-efficiency antimicrobial is essential for the management of severe bleeding wounds. Cotton dressings are widely used in clinical practice; however, few dressings can simultaneously achieve rapid hemostasis and high-efficacy antimicrobial. Herein, a versatile cotton dressing (GCQCNF-5) was masterly developed by sequentially deposition catechol modified quaternary chitosan (CQCS) and gelatin onto a cotton nonwoven fabric (NF) surface. Because of the presence of a gelatin sponge layer with suitable thickness and porosity on its surface, GCQCNF-5 exhibits a notably enhanced hemostatic effect compared to NF both in vitro and in vivo, which is even slightly superior to that of MHS (a commercial gelatin hemostatic sponge). Moreover, after exerting a rapid hemostasis effect, GCQCNF-5 can further exploit CQCS to exert excellent immediate antimicrobial and long-lasting bacteriostatic effects. In vivo wound healing experiments further indicated that GCQCNF-5 could significantly promote rapid healing of infected wounds by effectively sterilizing, absorbing exudates, and providing moist healing environments. This study clearly demonstrated that GCQCNF-5 is a versatile wound dressing that can achieve simultaneously rapid hemostasis, high-efficiency antimicrobial, and promote rapid healing of infected wounds, with great application potential for the treatment of severe bleeding wounds.