The TGEV Membrane Protein Interacts with HSC70 To Direct Virus Internalization through Clathrin-Mediated Endocytosis
Zhaoyang Ji, Hui Dong, Ruixue Jiao, Xiaoyuan Zhu, Hongyan Shi, Jianfei Chen, Da Shi, Jianbo Liu, Zhaoyang Jing, Jialin Zhang, Xiaobo Wang, Dandan Ye, Jiyu Zhang, Xin Zhang, Li Feng
Abstract
TGEV is the causative agent of porcine diarrhea, a viral disease that economically affects the pig industry in many countries. However, the molecular mechanisms underlying viral replication remain incompletely understood. Here, we provide evidence of a previously undescribed role of M protein in viral replication during early stages. We also identified HSC70 as a new host factor affecting TGEV infection. We demonstrate that the interaction between M and HSC70 directs TGEV internalization in a manner dependent on CME, thus revealing a novel mechanism for TGEV replication. We believe that this study may change our understanding of the first steps of infection of cells with coronavirus. This study should facilitate the development of anti-TGEV therapeutic agents by targeting the host factors and may provide a new strategy for the control of porcine diarrhea.