Clinical Correlation of Adenoviral Load in the Respiratory Tract Measured by Digital PCR in Immunocompromised Children
Diego R. Hijano, Jose Ferrolino, Jennifer Hidinger, Jessica N. Brazelton, Zhengming Gu, Sri Suganda, Heather L. Glasgow, Kim Allison, Megan Peterson, Ronald H. Dallas, Gabriela Marón, Randall T. Hayden
Abstract
Immunocompromised patients can have life-threatening adenoviral infection. Viral load in blood and stool is commonly used to guide antiviral therapy. We developed and evaluated a digital polymerase chain reaction assay to quantify human adenovirus in the respiratory tract and showed that higher peak load correlates with disseminated infection, mechanical ventilation, and death.
Topics & Concepts
MedicineRespiratory tractCorrelationRespiratory tract infectionsViral loadRespiratory systemVirologyIntensive care medicineImmunologyInternal medicineHuman immunodeficiency virus (HIV)GeometryMathematicsRespiratory viral infections researchVirus-based gene therapy researchViral gastroenteritis research and epidemiology