A global study for acute myeloid leukemia with <i>RARG</i> rearrangement
Hong‐Hu Zhu, Ya‐Zhen Qin, Zhanglin Zhang, Yongjing Liu, Lijun Wen, M. James You, Cheng Zhang, Esperanza Such, Hong Luo, Hongjian Yuan, Hongsheng Zhou, Hongxing Liu, Reng Xu, Ji Li, Jianhu Li, Hao Jian-ping, Jie Jin, Liang Yu, Jingying Zhang, Li-Ping Liu, Leping Zhang, Ruibin Huang, Shuhong Shen, Sujun Gao, Wei Wang, Xiaojing Yan, Xinyou Zhang, Xin Du, Xiaoxia Chu, Yanfang Yu, Yi-Xiang Wang, Yingchang Mi, Ying Lu, Zhen Cai, Zhan Su, David Taussig, Suzanne MacMahon, Edward D. Ball, Huan‐You Wang, John S. Welch, C. Cameron Yin, Gautam Borthakur, Miguel Á. Sanz, Hagop M. Kantarjian, Jinyan Huang, Jiong Hu, Suning Chen
Abstract
Acute myeloid leukemia (AML) with retinoic acid receptor γ (RARG) rearrangement has clinical, morphologic, and immunophenotypic features similar to classic acute promyelocytic leukemia. However, AML with RARG rearrangement is insensitive to alltrans retinoic acid (ATRA) and arsenic trioxide (ATO) and carries a poor prognosis. We initiated a global cooperative study to define the clinicopathological features, genomic and transcriptomic landscape, and outcomes of AML with RARG rearrangements collected from 29 study groups/institutions worldwide. Thirty-four patients with AML with RARG rearrangements were identified. Bleeding or ecchymosis was present in 18 (54.5%) patients. Morphology diagnosed as M3 and M3v accounted for 73.5% and 26.5% of the cases, respectively. Immunophenotyping showed the following characteristics: positive for CD33, CD13, and MPO but negative for CD38, CD11b, CD34, and HLA-DR. Cytogenetics showed normal karyotype in 38% and t(11;12) in 26% of patients. The partner genes of RARG were diverse and included CPSF6, NUP98, HNRNPc, HNRNPm, PML, and NPM1. WT1- and NRAS/KRAS-mutations were common comutations. None of the 34 patients responded to ATRA and/or ATO. Death within 45 days from diagnosis occurred in 10 patients (∼29%). At the last follow-up, 23 patients had died, and the estimated 2-year cumulative incidence of relapse, event-free survival, and overall survival were 68.7%, 26.7%, and 33.5%, respectively. Unsupervised hierarchical clustering using RNA sequencing data from 201 patients with AML showed that 81.8% of the RARG fusion samples clustered together, suggesting a new molecular subtype. RARG rearrangement is a novel entity of AML that confers a poor prognosis. This study is registered with the Chinese Clinical Trial Registry (ChiCTR2200055810).