Metal- and Affinity-Specific Dual Labeling of Cysteine-Rich Proteins for Identification of Metal-Binding Sites
Manuel David Peris‐Díaz, Roman Guráň, Ondřej Zítka, Vojtěch Adam, Artur Krężel
Abstract
MT species using a bottom-up MS approach with respect to metal-to-protein affinity and element(al) resolution. The methodology presented might be applied not only for MT2 but to identify metal-binding sites in other Cys-containing proteins.
Topics & Concepts
ChemistryIodoacetamideMetallothioneinCysteineMetalAlkylationCombinatorial chemistryBinding siteZincReagentBiochemistryOrganic chemistryEnzymeCatalysisTrace Elements in HealthIron Metabolism and DisordersHeavy Metal Exposure and Toxicity