Change in Cofactor Specificity of Oxidoreductases by Adaptive Evolution of an Escherichia coli NADPH-Auxotrophic Strain
Madeleine Bouzon, Volker Döring, Ivan Dubois, Anne Berger, Gabriele Stoffel, Liliana Calzadiaz Ramirez, Sophia N. Meyer, Marion Fouré, David Roche, Alain Perret, Tobias J. Erb, Arren Bar‐Even, Steffen N. Lindner
Abstract
In the cell, NAD(H) and NADP(H) cofactors have different functions. The former mainly accepts electrons from catabolic reactions and carries them to respiration, while the latter provides reducing power for anabolism.
Topics & Concepts
CofactorDehydrogenaseNAD+ kinaseBiologyAuxotrophyEnzymeBiochemistryEscherichia coliOxidoreductaseAlcohol dehydrogenaseStrain (injury)GeneAnatomyMicrobial Metabolic Engineering and BioproductionMitochondrial Function and PathologyEnzyme Structure and Function