Single-Cell Mass Spectrometry Enables Insight into Heterogeneity in Infectious Disease
Tra D. Nguyen, Yunpeng Lan, Shelley S. Kane, Jacob J. Haffner, Renmeng Liu, Laura‐Isobel McCall, Zhibo Yang
Abstract
infection in vitro. Our results showed that parasite-infected cells possessed divergent metabolism compared to control cells. Strikingly, some uninfected cells adjacent to infected cells showed metabolic impacts as well. Specific metabolic changes include increases in glycerophospholipids with infection. These results provide novel insight into the pathogenesis of CD. Furthermore, they represent the first application of bioanalytical SCMS to the study of mammalian-infectious agents, with the potential for broad applications to study infectious diseases.
Topics & Concepts
Trypanosoma cruziChagas diseaseMetabolomicsBiologySingle-cell analysisPathogenesisInfectious disease (medical specialty)CellDiseasePathogenMicrobiologyParasite hostingVirologyImmunologyGeneticsBioinformaticsMedicineComputer sciencePathologyWorld Wide WebTrypanosoma species research and implicationsBiochemical and Molecular ResearchResearch on Leishmaniasis Studies