The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts
D. O. Bazhenov, V. A. Mikhailova, Igor P. Nikolayenkov, K. L. Markova, Zeina Salloum, Igor Yu. Kogan, Aleksandr M. Gzgzyan, S. А. Selkov, Д. И. Соколов
Abstract
OBJECTIVE: The present study was to estimate the role of cytokines for trophoblast death in NK cells presence. METHODS: This study involves assessment of NK-92 line NK cell cytotoxic activity against JEG-3 line cells, in presence of cytokines. We also assessed the effect of secretory placenta products on NK cell cytotoxic activity toward JEG-3 line cells. RESULTS: Uteroplacental contact zone cytokines are able to enhance trophoblast mortality both by themselves in case of IL-1β, IL-6, IFNγ, IL-4, TGFβ, bFGF, and also through increasing the cytotoxic potential of NK cells in case of IL-1β, IFNγ, IL-8, TGFβ, and GM-CSF. PLGF decreases NK cell cytotoxicity for trophoblasts. Secretory products of first trimester placenta enhance NK cell cytotoxic potential for trophoblasts. CONCLUSIONS: Cytokines of the uteroplacental contact zone can appear a mechanism ensuring trophoblast mortality dynamics throughout pregnancy.