Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA)
Stephan Sinn, Joana Krämer, Frank Biedermann
Abstract
A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests.
Topics & Concepts
Displacement (psychology)ChemistryPsychologyPsychotherapistMolecular spectroscopy and chiralitySupramolecular Chemistry and ComplexesMolecular Sensors and Ion Detection