Green Synthesis of Trimetallic Nanocomposite (Ru/Ag/Pd)-Np and Its In Vitro Antimicrobial and Anticancer Activities
Shaimaa Hussein, Ayman M. Mahmoud, Hassan A. Elgebaly, Omnia Magdy Hendawy, Emad H. M. Hassanein, Shaima M. N. Moustafa, Nasser F. Alotaibi, Amr Nassar
Abstract
In this study, we used the aqueous extract of garlic tunicate leaf to reduce a mixture of equal amounts of ruthenium chloride, silver nitrate, and palladium acetate for the biosynthesis of ruthenium/silver/palladium trimetallic nanocomposite (Ru/Ag/Pd)-Np. Some physicochemical tools were used for nanocomposite characterization, including Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermal gravimetric analysis (TGA), UV-Vis spectroscopy (UV-Vis), scanning electron microscope (SEM), and transmittance electron microscope (TEM). XRD revealed that the crystal size of the nanocomposite is 15.67 nm. The TEM images showed that the particle size ranged 50–90 nm. The antimicrobial efficacy of the nanocomposite was examined against Aspergillus flavus, Aspergillus niger, Candida albicans, Candida glabrata, Escherichia coli, and Bacillus cereus. The results showed a potent antimicrobial activity toward all tested microorganisms. (Ru/Ag/Pd)-Np showed antiproliferative activity against Caco-2, HepG2, and K562 cell lines. The antiproliferative potential of (Ru/Ag/Pd)-Np was significantly improved following UV irradiation.