Human adipose and skeletal muscle tissue DNA, RNA, and protein content
Andrew M. Stroh, Colleen E. Lynch, Bridget Lester, Kiril Minchev, Toby L. Chambers, Cristhian F. Montenegro, Clarisa Chavez, William A. Fountain, Todd A. Trappe, Scott Trappe
Abstract
This investigation studied DNA, RNA, and protein contents of adipose and skeletal muscle tissues from young active individuals. A series of optimization steps were investigated to aid in determining the optimal approach to extract high-yield and high-quality biomolecules. These findings contribute to the knowledge gap in adipose tissue requirements for molecular biology assays, which is of increasing importance due to the growing interest in adipose tissue research involving human exercise physiology research.
Topics & Concepts
Skeletal muscleAdipose tissueRNADNAMuscle tissueBiologyAnatomyChemistryEndocrinologyCell biologyBiochemistryGeneAdipose Tissue and MetabolismExercise and Physiological ResponsesMuscle metabolism and nutrition