On-chip integrated graphene aptasensor with portable readout for fast and label-free COVID-19 detection in virus transport medium
Lizhou Xu, Sami Ramadan, Bruno M. G. Rosa, Yuanzhou Zhang, Tianyi Yin, Elias Torres, Olena Shaforost, Apostolos Panagiotopoulos, Bing Li, Gwilherm Kerherve, Dong Kuk Kim, Cecilia Mattevi, Long R. Jiao, Peter K. Petrov, N. Klein
Abstract
particles per mL for virus-like particles (VLPs) in clinical transport medium, outperforming the Ab-GFET biosensor counterpart. In addition, the aptasensor achieved a LOD of 160 aM for COVID-19 neutralizing antibodies in serum. The sensors were found to be highly selective, fast (sample-to-result within minutes), and stable (low device-to-device signal variation; relative standard deviations below 0.5%). A home-built portable readout electronic unit was employed for simultaneous real-time measurements of 12 GFETs per chip. Our successful demonstration of a portable GFET biosensing platform has high potential for infectious disease detection and other health-care applications.