MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B
Hongxing Li, Zhi‐Ling Li, Yigang Pi, Yang Chen, Lin Mei, Yong Luo, Jingping Xie, Xinzhan Mao
Abstract
OBJECTIVE: This study aimed to explore the underlying mechanism of miR-375 in exacerbating osteoarthritis (OA). RESULTS: results further verified that inhibition of miR-375 could relieve OA-related symptoms. CONCLUSION: miR-375 can inhibit the expression of ATG2B in chondrocytes, suppress autophagy and promote the ERs. It suggests that miR-375 could be considered to be a key therapy target for OA. METHODS: , OA mice model was established, HE and Safranin O and Fast Green staining, as well as the OARSI and modified Mankin scores, were applied to measure the OA cartilage damage severity.
Topics & Concepts
ChondrocyteAutophagymicroRNAOsteoarthritisWestern blotIn vivoDownregulation and upregulationCartilageApoptosisEndoplasmic reticulumFlow cytometryCell biologyTransfectionChemistryCancer researchBiologyMolecular biologyMedicinePathologyGeneBiochemistryAnatomyAlternative medicineBiotechnologyOsteoarthritis Treatment and MechanismsCancer-related molecular mechanisms researchExtracellular vesicles in disease