Targeted A-to-G base editing of chloroplast DNA in plants
Young Geun Mok, Sunghyun Hong, Su‐Ji Bae, Sung-Ik Cho, Jin‐Soo Kim
Abstract
Abstract Chloroplast DNA (cpDNA) encodes up to 315 (typically, 120–130) genes 1 , including those for essential components in photosystems I and II and the large subunit of RuBisCo, which catalyses CO 2 fixation in plants. Targeted mutagenesis in cpDNA will be broadly useful for studying the functions of these genes in molecular detail and for developing crops and other plants with desired traits. Unfortunately, CRISPR–Cas9 and CRISPR-derived base editors, which enable targeted genetic modifications in nuclear DNA, are not suitable for organellar DNA editing 2 , owing to the difficulty of delivering guide RNA into organelles. CRISPR-free, protein-only base editors (including DddA-derived cytosine base editors 3–8 and zinc finger deaminases 9 ), originally developed for mitochondrial DNA editing in mammalian cells, can be used for C-to-T, rather than A-to-G, editing in cpDNA 10–12 . Here we show that heritable homoplasmic A-to-G edits can be induced in cpDNA, leading to phenotypic changes, using transcription activator-like effector-linked deaminases 13 .