HSPB1 overexpression improves hypoxic-ischemic brain damage by attenuating ferroptosis in rats through promoting G6PD expression
Yi Dai, Lan Hu
Abstract
HSPB1 and G6PD were overexpressed in the hippocampus tissues of HI rats. High expression of HSPB1 in HI rats mitigated infarction rate and iron accumulation. HSPB1 overexpression reduced ferritin level, attenuated apoptosis, yet augmented GPX4, SLC7A11, and TFR1 levels in the hippocampus tissues of HI rats. G6PD deletion impaired the protective role of HSPB1 overexpression against HI brain damage-induced ferroptosis.
Topics & Concepts
NeuroprotectionTUNEL assayViability assayApoptosisWestern blotDownregulation and upregulationChemistryBrain damageMTT assayHippocampal formationReactive oxygen speciesMolecular biologyPharmacologyBiologyBiochemistryEndocrinologyNeuroscienceGeneHeme Oxygenase-1 and Carbon MonoxideMicroRNA in disease regulationMitochondrial Function and Pathology