Deciphering Cellodextrin and Glucose Uptake in <i>Clostridium thermocellum</i>
Fei Yan, Sheng Dong, Yajun Liu, Xingzhe Yao, Chao Chen, Yan Xiao, Edward A. Bayer, Yuval Shoham, Chun You, Qiu Cui, Yingang Feng
Abstract
Highly efficient sugar uptake is important to microbial cell factories, and sugar transporters are therefore of great interest in the study of industrially relevant microorganisms. Clostridium thermocellum is a lignocellulolytic bacterium known for its multienzyme complex, the cellulosome, which is of great potential value in lignocellulose biorefinery. In this study, we clarify the function and mechanism of substrate specificity of the five reported putative sugar transporters using genetic, biophysical, and structural methods.
Topics & Concepts
Clostridium thermocellumBiochemistryATP-binding cassette transporterCellulosomeTransporterThermophileATPaseCellobioseChemistryBiologyCellulaseCelluloseGeneEnzymeBiofuel production and bioconversionAdvanced Cellulose Research StudiesMicrobial Metabolic Engineering and Bioproduction