Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer
Phimmada Hatthakarnkul, Aula Ammar, Kathryn A.F. Pennel, Leah Officer-Jones, Silvia Cusumano, Jean A. Quinn, Amna Ahmed Mohemmed Matly, Peter Alexander, Jennifer Hay, Ditte K. Andersen, Gerard Lynch, Hester C. van Wyk, Noori Maka, Donald McMillan, John Le Quesne, Chanitra Thuwajit, Joanne Edwards
Abstract
PURPOSE: Colorectal cancer (CRC) is the third most diagnosed cancer worldwide. Despite a well-established knowledge of tumour development, biomarkers to predict patient outcomes are still required. S100 calcium-binding protein A2 (S100A2) has been purposed as a potential marker in many types of cancer, however, the prognostic value of S100A2 in CRC is rarely reported. MATERIAL AND METHODS: In this study, immunohistochemistry (IHC) was performed to identify the prognostic role of S100A2 protein expression in the tumour core of the tissue microarrays (TMAs) in colorectal cancer patients (n=787). Bulk RNA transcriptomic data was used to identify significant genes compared between low and high cytoplasmic S100A2 groups. Multiplex immunofluorescence (mIF) was performed to further study and confirm the immune infiltration in tumours with low and high cytoplasmic S100A2. RESULTS: =0.009 respectively). CONCLUSION: Our finding demonstrates a prognostic value of S100A2 together with the correlation with immune infiltration in CRC.