A self-assembling peptidic platform to boost the cellular uptake and nuclear delivery of oligonucleotides
Shabnam Tarvirdipour, Michal Skowicki, Cora‐Ann Schoenenberger, Larisa E. Kapinos, Roderick Y. H. Lim, Yaakov Benenson, Cornelia G. Palivan
Abstract
the NLS interaction with the nuclear transport receptors of the karyopherin family. Importantly, we observed that the treatment of MCF-7 cells with NLS-MCMs loaded with anti-BCL2 antisense oligonucleotides resulted in up to 86% knockdown of BCL2, an inhibitor of apoptosis that is overexpressed in more than half of all human cancers. We envision that this platform can be used to efficiently entrap and deliver diverse genetic payloads to the nucleus and find applications in basic research and biomedicine.
Topics & Concepts
OligonucleotideDNANucleusChemistryCell nucleusCellCell biologyComputational biologyNanotechnologyBiophysicsBiochemistryBiologyMaterials scienceAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry