Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
Qianqian Qi, Yutong Zhang, Wei Xiong, Xingyu Liu, Shuangyu Cui, Xiaofang Ye, Kaisong Zhang, Tian Tian, Xiang Zhou
Abstract
In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished.
Topics & Concepts
TetrazineChemistryCRISPRLigationComputational biologyRNABiologyBiochemistryMolecular biologyOrganic chemistryGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesClick Chemistry and Applications