Drought re-routes soil microbial carbon metabolism towards emission of volatile metabolites in an artificial tropical rainforest
Linnea K. Honeker, Giovanni Pugliese, Johannes Ingrisch, Jane Fudyma, Juliana Gil-Loaiza, Elizabeth Carpenter, Esther Singer, Gina Hildebrand, Lingling Shi, David Hoyt, Rosalie Chu, Jason Toyoda, Jordan Krechmer, Megan S. Claflin, Christian Ayala-Ortiz, Viviana Freire-Zapata, Eva Y. Pfannerstill, L. Erik Daber, Kathiravan Meeran, Michaela A. Dippold, Jürgen Kreuzwieser, Jonathan Williams, S. Nemiah Ladd, Christiane Werner, Malak Tfaily, Laura Meredith
Abstract
Abstract Drought impacts on microbial activity can alter soil carbon fate and lead to the loss of stored carbon to the atmosphere as CO 2 and volatile organic compounds (VOCs). Here we examined drought impacts on carbon allocation by soil microbes in the Biosphere 2 artificial tropical rainforest by tracking 13 C from position-specific 13 C-pyruvate into CO 2 and VOCs in parallel with multi-omics. During drought, efflux of 13 C-enriched acetate, acetone and C 4 H 6 O 2 (diacetyl) increased. These changes represent increased production and buildup of intermediate metabolites driven by decreased carbon cycling efficiency. Simultaneously, 13 C-CO 2 efflux decreased, driven by a decrease in microbial activity. However, the microbial carbon allocation to energy gain relative to biosynthesis was unchanged, signifying maintained energy demand for biosynthesis of VOCs and other drought-stress-induced pathways. Overall, while carbon loss to the atmosphere via CO 2 decreased during drought, carbon loss via efflux of VOCs increased, indicating microbially induced shifts in soil carbon fate.