Novel DEK-Targeting Aptamer Delivered by a Hydrogel Microneedle Attenuates Collagen-Induced Arthritis
Jian Cao, Jingjing Su, Mengchen An, Yang Yang, Yi Zhang, Jing Zuo, Nan Zhang, Yongxing Zhao
Abstract
. DTA4 was then modified with cholesterol on its 5' end to form DTA6. DTA6 dramatically reduces DEK expression in inflammatory RAW264.7 cells. A hydrogel microneedle (hMN) was then fabricated for the transdermal delivery of DTA6. The hMN maintains morphological integrity after absorbing the aptamer solution, effectively pierces the skin, and rapidly releases DTA6 into the dermis. The DTA6-loaded hMN significantly attenuates inflammation and protects joints from cartilage/bone erosion in collagen-induced arthritis (CIA) mice.
Topics & Concepts
ChemistryIn vivoInflammationNucleaseArthritisRheumatoid arthritisTransdermalBiophysicsAptamerIn vitroPharmacologyCartilageCell biologyBiochemistryMolecular biologyImmunologyMedicineAnatomyEnzymeBiotechnologyBiologyCell Adhesion Molecules ResearchNeutrophil, Myeloperoxidase and Oxidative MechanismsErythrocyte Function and Pathophysiology