Simple and sensitive spectrophotometric method for estimating the nitrogen-fixing capacity of bacterial cultures
A. Cordova-Rodriguez, María Eugenia Rentería-Martínez, Claudio A. Lopez-Miranda, Juan Manuel Guzmán-Ortiz, Sergio Francisco Moreno-Salazar
Abstract
is the nitrogen concentration in the culture medium.•The methods used at present to measure the efficiency of BNF require expensive equipment, which may not be affordable for many laboratories or companies working in this field.•This technique can be used for pure bacterial strains and microbial consortia from soil or commercial products.
Topics & Concepts
Bromothymol blueAbsorbanceDiazotrophNitrogenAmmoniumMicroorganismNitrogen fixationPhotobioreactorAmmoniapH indicatorChemistryEnvironmental chemistryEnvironmental scienceBacteriaChromatographyBiologyAgronomyBiochemistryOrganic chemistryBiomass (ecology)GeneticsWastewater Treatment and Nitrogen RemovalMicrobial Fuel Cells and BioremediationLegume Nitrogen Fixing Symbiosis