Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome
Changjiang Fan, Xinyue Meng, Wei Yang, Peiyan Wang, Wenguang Chang, Peifeng Li, Jianxun Wang
Abstract
the click reaction, achieving 8-oxoGua pull-down and sequencing. Using APSC-8-oxoGua-seq, 8-oxoGua can be identified at single-base resolution.
Topics & Concepts
TranscriptomemicroRNABase (topology)Computational biologyChemistryBiologyGeneticsGeneGene expressionMathematicsMathematical analysisRNA modifications and cancerMicroRNA in disease regulationRNA and protein synthesis mechanisms