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Metagenomics of Wastewater Influent from Southern California Wastewater Treatment Facilities in the Era of COVID-19

Jason A. Rothman, Theresa B. Loveless, Madison Griffith, Joshua A. Steele, John F. Griffith, Katrine Whiteson

2020Microbiology Resource Announcements17 citationsDOIOpen Access PDF

Abstract

Sequencing wastewater may be useful for detecting pathogens and assaying microbial water quality. We concentrated, extracted, and sequenced nucleic acids from 17 composite influent wastewater samples spanning seven southern California wastewater treatment facilities in May 2020. Bacteria were the most proportionally abundant taxonomic group present, followed by viruses and archaea.

Topics & Concepts

WastewaterMetagenomicsSewage treatmentArchaeaCoronavirus disease 2019 (COVID-19)BiologyEnvironmental scienceSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)BacteriaEnvironmental engineeringMedicineInfectious disease (medical specialty)GeneDiseasePathologyBiochemistryGeneticsSARS-CoV-2 detection and testingBiosensors and Analytical DetectionCOVID-19 diagnosis using AI