Development and verification of sex‐specific molecular marker for Golden pompano ( <i>Trachinotus blochii</i> )
Kaixi Zhang, Feibiao Song, Yesong Liang, Weiwei Zhang, Kexin Zhou, Youming Chen, Peng Zhen, Jian Luo
Abstract
There are common significant character differences between genders in many fish. The sex-specific genetic markers are important tools for research and industry. Golden pompano (Trachinotus blochii) is a commercial mass-produced species with sex growth differences. Our study includes the whole genome re-sequencing data of T. blochii from four populations. A total of 2,183,486 high-quality clean reads were obtained for subsequent information analysis. Association analysis of single nucleotide polymorphism markers with sex was carried out, the genome-wide association studyGWAS was carried out using the simple linear regression model (Q), and a high-scored site loci in LG20 exhibiting significant association with sex was found. A heterozygous mutation was observed in this site in female fish (G/A), and a homozygous mutation in males (A/A). Within the 50kb annotation interval of this variant site, hsd17b1 was found to be linked to sex-related pathways significantly. Based on the site, we developed a simple and efficient polymerase chain reaction-based sex-specific molecular marker for identifying the T. blochii genetics sex. It will benefit the sex-controlled breeding and sex development study of the golden pompano.