Protocol for generation of human peptide-specific primary CD8+ T cell lines
Emma J. Grant, Stéphanie Gras
Abstract
Directly ex vivo, peptide-specific CD8+ T cells are present at relatively low frequency and are typically in a resting state. This protocol details the expansion of memory peptide-specific CD8+ T cells by in vitro stimulation, which can be subsequently characterized using a range of assays including tetramer staining and intracellular cytokine staining. For complete details on the use and execution of this protocol, please refer to Lineburg et al. (2021).
Topics & Concepts
Ex vivoCD8Cytotoxic T cellIntracellularPeptideCell biologyIn vitroChemistryProtocol (science)BiologyMolecular biologyImmunologyMedicineImmune systemBiochemistryPathologyAlternative medicineImmunotherapy and Immune ResponsesImmune Cell Function and InteractionCAR-T cell therapy research