Programmable adenine deamination in bacteria using a Cas9–adenine-deaminase fusion
Ya Zhang, Hongyuan Zhang, Zhipeng Wang, Zhaowei Wu, Yu Wang, Na Tang, Xuexia Xu, Suwen Zhao, Weizhong Chen, Quanjiang Ji
Abstract
We report a pABE system which enables highly efficient adenine to guanine conversion in bacteria. Key residues of a staphylopine/metal complex transporter cntBC were systematically screened via the pABE system.
Topics & Concepts
DeaminationFusionChemistryCombinatorial chemistryBiochemistryComputer scienceComputational biologyBiologyEnzymePhilosophyLinguisticsCRISPR and Genetic EngineeringVirus-based gene therapy researchRNA and protein synthesis mechanisms