A Genome-Wide Association Study Identifies Novel Susceptibility <i>loci</i> in Chronic Chagas Cardiomyopathy
Desiré Casares‐Marfil, Mariana Strauss, Pau Bosch‐Nicolau, María Silvina Lo Presti, Israel Molina, Christophe Chevillard, Edécio Cunha‐Neto, Éster Cerdeira Sabino, Antônio Luiz Pinho Ribeiro, Clara Isabel González, Javier Martı́n, Marialbert Acosta‐Herrera
Abstract
BACKGROUND: Chagas disease is an infectious disease caused by the parasite Trypanosoma cruzi and is endemic from Latin American countries. The goal of our study was to identify novel genetic loci associated with chronic Chagas cardiomyopathy development in Chagas disease patients from different Latin American populations. METHODS: We performed a cross-sectional, nested case-control study including 3 sample collections from Colombia, Argentina, and Bolivia. Samples were genotyped to conduct a genome-wide association study (GWAS). These results were meta-analyzed with summary statistic data from Brazil, gathering a total of 3413 Chagas disease patients. To identify the functional impact of the associated variant and its proxies, we performed an in silico analysis of this region. RESULTS: The meta-analysis revealed a novel genome-wide statistically significant association with chronic Chagas cardiomyopathy development in rs2458298 (OR = 0.90, 95%CI = 0.87-0.94, P-value = 3.27 × 10-08), nearby the SAC3D1 gene. In addition, further in silico analyses displayed functional relationships between the associated variant and the SNX15, BAFT2, and FERMT3 genes, related to cardiovascular traits. CONCLUSIONS: Our findings support the role of the host genetic factors in the susceptibility to the development of the chronic cardiac form of this neglected disease.