miR-31-5p as a Potential Circulating Biomarker and Tracer of Clinical Improvement for Chronic Inflammatory Demyelinating Polyneuropathy

Abstract

Background. MicroRNAs are endogenous, small noncoding RNA molecules that play a pivotal role in the regulation of gene expression. MicroRNAs are involved in many biological processes such as proliferation, cell differentiation, neovascularization, and apoptosis. Studies on microRNA expression may contribute to a better understanding of the pathomechanism of chronic inflammatory demyelinating polyneuropathy (CIDP) and consequently enable the development of new therapeutic measures using antisense miRNAs (antagomirs). In this study, we evaluated the level of miR-31-5p in the serum of patients with CIDP and its correlation with the miR-31-5p level and clinical presentation and electrophysiological and biochemical parameters. Methods. The study group consisted of 48 patients, mean age <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mn>61.60</a:mn> <a:mo>±</a:mo> <a:mn>11.76</a:mn> </a:math> , who fulfilled the diagnostic criteria of a typical variant of CIDP. The expression of miR-31-5p in patient serum probes was investigated by droplet digital PCR. The results were correlated with neurophysiological findings and the patient’s clinical and biochemical parameters. Results. The mean copy number of miRNA-31 in 100 μl serum was <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"> <c:mn>1288.64</c:mn> <c:mo>±</c:mo> <c:mn>2001.02</c:mn> </c:math> in the CIDP group of patients, while in the control group, it was <e:math xmlns:e="http://www.w3.org/1998/Math/MathML" id="M3"> <e:mn>3743.09</e:mn> <e:mo>±</e:mo> <e:mn>4026.90</e:mn> </e:math> . There was a significant positive correlation (0.426) between IgIV treatment duration and miR-31-5p expression. Patients without IgIV treatment showed significantly lower levels of miR-31 compared to the treated group ( <g:math xmlns:g="http://www.w3.org/1998/Math/MathML" id="M4"> <g:mn>259.44</g:mn> <g:mo>±</g:mo> <g:mn>304.02</g:mn> </g:math> vs. <i:math xmlns:i="http://www.w3.org/1998/Math/MathML" id="M5"> <i:mn>1559.48</i:mn> <i:mo>±</i:mo> <i:mn>2168.45</i:mn> </i:math> ; <k:math xmlns:k="http://www.w3.org/1998/Math/MathML" id="M6"> <k:mi>p</k:mi> <k:mo>=</k:mo> <k:mn>0.002</k:mn> </k:math> ). The group of patients with <m:math xmlns:m="http://www.w3.org/1998/Math/MathML" id="M7"> <m:mtext>body</m:mtext> <m:mtext> </m:mtext> <m:mtext>weight</m:mtext> <m:mo>&gt;</m:mo> <m:mn>80</m:mn> <m:mtext> </m:mtext> <m:mtext>kg</m:mtext> </m:math> showed statistically significantly lower levels of miRNA-31-5p than the patients with lower body weight ( <o:math xmlns:o="http://www.w3.org/1998/Math/MathML" id="M8"> <o:mn>934.37</o:mn> <o:mo>±</o:mo> <o:mn>1739.66</o:mn> </o:math> vs. <q:math xmlns:q="http://www.w3.org/1998/Math/MathML" id="M9"> <q:mn>1784.62</q:mn> <q:mo>±</q:mo> <q:mn>2271.62</q:mn> </q:math> , respectively; <s:math xmlns:s="http://www.w3.org/1998/Math/MathML" id="M10"> <s:mi>p</s:mi> <s:mo>=</s:mo> <s:mn>0.014</s:mn> </s:math> ). Similarly, the patients with elevated cerebrospinal fluid (CSF) protein levels had significantly higher miRNA-31-5p expression than those with normal protein levels ( <u:math xmlns:u="http://www.w3.org/1998/Math/MathML" id="M11"> <u:mn>1393.93</u:mn> <u:mo>±</u:mo> <u:mn>1932.27</u:mn> </u:math> vs. <w:math xmlns:w="http://www.w3.org/1998/Math/MathML" id="M12"> <w:mn>987.38</w:mn> <w:mo>±</w:mo> <w:mn>2364.10</w:mn> </w:math> , respectively; <y:math xmlns:y="http://www.w3.org/1998/Math/MathML" id="M13"> <y:mi>p</y:mi> <y:mo>=</y:mo> <y:mn>0.044</y:mn> </y:math> ). Conclusion. The results may support the hypothesis that miR-31-5p is strongly involved in the autoimmune process in CIDP. The positive correlation between higher miR-31-5p levels and duration of IVIg treatment may be an additional factor explaining the efficacy of prolonged IVIg therapy in CIDP.

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