Stromal Cells Covering Omental Fat-Associated Lymphoid Clusters Trigger Formation of Neutrophil Aggregates to Capture Peritoneal Contaminants
Lucy H. Jackson‐Jones, Peter Smith, Jordan R. Portman, Marlène Magalhaes Pinto, Katie J. Mylonas, Matthieu Vermeren, Mark Nixon, Beth Emily Pollot Henderson, Ross Dobie, Sonja Vermeren, Laura Denby, Neil C. Henderson, Damian J. Mole, C Bénézech
Abstract
mesothelial cells, which we termed FALC cover cells. Blockade of CXCL1 inhibited the recruitment and aggregation of neutrophils at FALCs during zymosan-induced peritonitis. Inhibition of protein arginine deiminase 4, an enzyme important for the release of neutrophil extracellular traps, abolished neutrophil aggregation and the capture of peritoneal contaminants by omental FALCs. Analysis of omental samples from patients with acute appendicitis confirmed neutrophil recruitment and bacterial capture at FALCs. Thus, specialized omental mesothelial cells coordinate the recruitment and aggregation of neutrophils to capture peritoneal contaminants.