Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart
Savaş Üstünova, Selçuk Takır, Nadim Yılmazer, Huri Bulut, Didem Altındirek, Özden Hatırnaz Ng, Cihan Demirci Tansel, B. SONMEZ UYDES DOGAN, Uğur Özbek, Elif İlkay Armutak, Ebru Gürel Gürevin
Abstract
Background/Aim: This study was designed to provide further evidence for the interactions between hydrogen sulfide (H<sub>2</sub>S) and nitric oxide (NO) in ischemia/reperfusion (I/R) injury. Materials and Methods: Rat hearts were studied with the Langendorff technique using the H<sub>2</sub>S donor sodium hydrosulfide (NaHS, 40 μM) and the cystathionine gamma-lyase (CTH or CSE) inhibitor DL-propargylglycine (PAG, 1 mM). NO synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 30 mg/kg, 7 days) was administered before the isolation. The hearts were homogenized for biochemical and molecular analysis. Results: NaHS reversed I/R-induced cardiac performance impairment, increased tissue nitric oxide production and decreased tissue markers for cardiac injury, while L-NAME inhibited these effects. The expression of CTH was increased with PAG, which was suppressed by L-NAME. Conclusion: H<sub>2</sub>S and NO increase each other9s production suggesting their interaction and cooperation in cardioprotection against I/R injury.