Encapsulation state of messenger RNA inside lipid nanoparticles
Mark L. Brader, Sean Williams, Jessica M. Banks, Wong H. Hui, Z. Hong Zhou, Lin Jin
Abstract
Understanding the structure of messenger RNA (mRNA) lipid nanoparticles, and specifically the microenvironment of the mRNA molecules within these entities, is fundamental to advancing their biomedical potential. Here, we show that a permeating cationic dye, thionine, can serve as a cryogenic electron microscopy contrasting agent by binding selectively to encapsulated mRNA without disturbing lipid nanoparticle morphology. Cryo-electron microscopy images identify the mRNA location, revealing that mRNA may exist within solvent-filled cavities or may be substantially lipid associated.
Topics & Concepts
Messenger RNANanoparticleBiophysicsRNAChemistryCell biologyNanotechnologyMaterials scienceBiochemistryBiologyGeneRNA Interference and Gene DeliveryLipid Membrane Structure and BehaviorRNA and protein synthesis mechanisms