Pleiotropic Effects of Influenza H1, H3, and B Baloxavir-Resistant Substitutions on Replication, Sensitivity to Baloxavir, and Interferon Expression
Brady T. Hickerson, Simone E. Adams, Subrata Barman, Lance A. Miller, Vladimir Y. Lugovtsev, Richard J. Webby, William L. Ince, Raymond P. Donnelly, Natalia A. Ilyushina
Abstract
gene expression compared to the wild-type virus (4.2-fold). In contrast, the B variant, G199R, triggered the lowest levels of IFN genes in Calu-3 cells (1.6-fold). Because baloxavir is a novel anti-influenza therapeutic agent, identifying and characterizing substitutions associated with reduced sensitivity to baloxavir, as well as the impact of these substitutions on viral fitness, is paramount to the strategic implementation of this novel countermeasure.
Topics & Concepts
BiologyViral replicationVirusVirologyRecombinant DNAInfluenza A virusInterferonOrthomyxoviridaeMolecular biologyIn vitroPolymeraseGeneRibonucleoproteinCell cultureGeneticsRNAInfluenza Virus Research Studiesinterferon and immune responsesRespiratory viral infections research