Development of an MSPQC Nucleic Acid Sensor Based on CRISPR/Cas9 for the Detection of <i>Mycobacterium tuberculosis</i>
Ji Huang, Zi Liang, Yu Liu, Jiandang Zhou, Fengjiao He
Abstract
as the target. The detection time was down to 2.3 h. The limit of detection (LOD) was 30 CFU/mL.
Topics & Concepts
CRISPRDNAChemistryNucleic acidHybridization probeDetection limitMycobacterium tuberculosisCas9Polymerase chain reactionComputational biologyNucleic acid detectionTuberculosisBiologyChromatographyGeneBiochemistryMedicinePathologyAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic EngineeringInnovative Microfluidic and Catalytic Techniques Innovation