Dipstick-based rapid nucleic acids purification and CRISPR/Cas12a-mediated isothermal amplification for visual detection of African swine fever virus
Siwenjie Qian, Yanju Chen, Cheng Peng, Xiaofu Wang, Hui Wu, Yang Che, Huanying Wang, Junfeng Xu, Jian Wu
Topics & Concepts
Nucleic acidLoop-mediated isothermal amplificationAmpliconChemistryDipstickDetection limitAfrican swine fever virusNucleic acid methodsChromatographyNucleic acid quantitationCRISPRNucleic acid detectionAnalytePolymerase chain reactionDNABiochemistryUrineGeneAnimal Disease Management and EpidemiologyCRISPR and Genetic EngineeringViral Infections and Immunology Research