Optimization of Ultrasonic-Assisted Extraction of Phenolic Compounds from Moringa Oleifera Leaves using Response Surface Methodology
Abdelhakim Benarima, Salah Eddine Laouini, Bachir Ben Seghir, Yassine Belaiche, Mohammed Ridha Ouahrani
Abstract
Moringa Oleifera, or in other words the tree of life is considered one of the most useful plants in the world because it’s rich in bioactive substances, which employing on Modern medical treatment, also can be used for many fields: food, pharmaceutical and cosmetics purposes. The present study consists of determine the optimal extraction conditions to improve the yield of the total phenolic content (TPC) from Moringa Oleifera leaves, by applied the response surface methodology (RSM) based on a Box-Behnken design (BBD) and the use of ultrasonic-assisted extraction as a new extraction technique. The results showed that the optimum extraction conditions were found at extraction time, 20 (min); extraction temperature, 42 (°C); solvent/solid ratio, 5 (ml/g), with the maximum yield of total phenolic content achieved in a quantity of 278.64 (mg GAE/g), which agreed with the predicted value 280.41 (mg GAE/g) of statistical method.