Backbone and methyl side-chain resonance assignments of the single chain Fab fragment of trastuzumab
Donald Gagné, James M. Aramini, Yves Aubin
Abstract
Trastuzumab is a therapeutic monoclonal antibody developed to target human epidermal growth factor receptor 2 (HER2) present at higher levels in early cancers. Here we report the near complete resonance assignment of trastuzumab-scFab fragment backbone and the methyl groups of isoleucine, leucine and valine residues, as well as their stereo-assignments. The antibody fragment was produced using a single chain approach in Escherichia coli.
Topics & Concepts
TrastuzumabValineMonoclonal antibodyIsoleucineChemistryFragment (logic)Side chainImmunoglobulin light chainAntibodyStereochemistryEscherichia coliMolecular biologyLeucineBiochemistryBiologyAmino acidGeneticsCancerGeneBreast cancerComputer scienceOrganic chemistryProgramming languagePolymerMonoclonal and Polyclonal Antibodies ResearchHER2/EGFR in Cancer ResearchGlycosylation and Glycoproteins Research