A new FRET probe for ratiometric fluorescence detecting mitochondria-localized drug activation and imaging endogenous hydroxyl radicals in zebrafish
Tao Deng, Xiaojuan Wang, Shengjun Wu, Shiyou Hu, Wei Liu, Tongkai Chen, Zhiqiang Yu, Qin Xu, Fang Liu
Abstract
A new FRET probe has been prepared for ratiometric fluorescence detection of hydroxyl radicals. It has been successfully used for detecting mitochondria-localized drug activation in living cells and imaging endogenous hydroxyl radicals in zebrafish gastrointestinal (GI) tracts under normal culturing conditions.
Topics & Concepts
RadicalFörster resonance energy transferChemistryEndogenyZebrafishFluorescencePhotochemistryHydroxyl radicalBiophysicsFluorescence-lifetime imaging microscopyBiochemistryBiologyGeneQuantum mechanicsPhysicsElectron Spin Resonance StudiesNitric Oxide and Endothelin EffectsMolecular Sensors and Ion Detection