Super-resolution re-scan second harmonic generation microscopy
Stefan G. Stanciu, Radu Hristu, George A. Stanciu, Denis E. Tranca, Lucian G. Eftimie, Adrian Dumitru, Mariana Costache, Harald Stenmark, Harm Manders, Amit Cherian, Mariliis Tark‐Dame, Erik M. M. Manders
Abstract
Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.