Gelatin/Nanofibrin bioactive scaffold prepared with enhanced biocompatibility for skin tissue regeneration
Rethinam Senthil, Sathyaraj Weslen Vedakumari, Santhosh Basavarajappa, Mohamed Hashem, Lakshmi Thangavelu, T. Senthilvelan, Serdar Batıkan Kavukcu, Baskar Venkidasamy, Ramachandran Vinayagam
Abstract
This research study was to develop a nano bioactive scaffold (NBAS) using gelatins (Gel), nanofibrin (Nano-FB), and glycerol (Gly) by film casting method for potential use in skin tissue engineering. The developed NBAS were characterized for their molecular interaction (Fourier transforms infrared spectroscopy (FTIR)), microstructure (scanning electron microscopy (S.E.M.)), mechanical strength (tensile strength (MPa), elongation at break (%), and flexibility (%)), and Cell viability (M.T.T. assay ((3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide)) were assessed in the biocompatibility study. The mechanical result of the N.B.F. possessed better (tensile strength of 5.22±0.07 MPa), (elongation at break of 5.88+0.04 %), and (flexibility of 9.18±0.09%) properties. The Invitro study using a human keratinocyte (HaCaT) cell line proved the 100% biocompatibility of NBAS. Based outcome of this study, performed its mechanical properties and exhibited biocompatibility in skin tissue engineering. The study has devised a process for using slaughterhouse and fish waste in the production of valuable medical products like wound dressing materials.