Rapid amplitude-modulation of a diarylethene photoswitch: en route to contrast-enhanced fluorescence imaging
Gaowa Naren, Wera Larsson, Carlos Benitez‐Martin, Shiming Li, Ezequiel Pérez‐Inestrosa, Bo Albinsson, Joakim Andréasson
Abstract
, the autofluorescence commonly observed in fluorescence microscopy. We show that this scheme is conveniently applicable using lock-in detection, and that robust amplitude modulation of the probe fluorescence is indeed possible also in cell studies using fluorescence microscopy.
Topics & Concepts
DiarylethenePhotoswitchFluorescenceContrast (vision)Modulation (music)Derivative (finance)Fluorescence-lifetime imaging microscopyPhotochemistryChemistryMaterials sciencePhotochromismOpticsPhysicsAcousticsFinancial economicsEconomicsPhotochromic and Fluorescence ChemistryPhotoreceptor and optogenetics researchAdvanced Fluorescence Microscopy Techniques