ELISA detection of SARS-CoV-2 antibodies in saliva
Melanie A. MacMullan, Albina Ibrayeva, Kylie Trettner, Laura Deming, Sudipta Sekhar Das, Frances Tran, Jose Ricardo Moreno, Joseph G. Casian, Prithivi Chellamuthu, Jeffrey Kraft, Kenneth H. Kozak, Fred Turner, Vladimir I. Slepnev, Lydia M. Le Page
Abstract
To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimizing exposure risk for healthcare workers.