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Design of a multi-epitope vaccine against SARS-CoV-2: immunoinformatic and computational methods

Md. Oliullah Rafi, Khattab Al-Khafaji, Md. Takim Sarker, Tuğba Taşkın‐Tok, Abdus Samad Rana, Md. Shahedur Rahman

2022RSC Advances58 citationsDOIOpen Access PDF

Abstract

identifying the key residues (named HUB nodes) that control interaction stability and provide a clear molecular mechanism. The obtained results from molecular docking and MD simulation revealed a strong and stable interaction between vaccine and TLRs. The vaccine's ability to stimulate the immune response was assessed by using computational immune simulation. This predicted a significant level of cytotoxic T cell and helper T cell activation, as well as IgG, interleukin 2, and interferon-gamma production. This study shows that the designed vaccine is structurally and dynamically stable and can trigger an effective immune response against viral infections.

Topics & Concepts

EpitopeVirologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Sars virusChemistryComputational biologyBiologyMedicineAntibodyImmunologyInfectious disease (medical specialty)DiseasePathologyvaccines and immunoinformatics approachesSARS-CoV-2 and COVID-19 ResearchMonoclonal and Polyclonal Antibodies Research
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