Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction in<i>Clostridium thermocellum</i>
Shuen Hon, Tyler B. Jacobson, David Stevenson, Marybeth Maloney, Richard J. Giannone, Robert L. Hettich, Daniel Amador‐Noguez, Daniel G. Olson, Lee R. Lynd
Abstract
. This study also identifies and overcomes a physiological hurdle toward expressing an ATP-dependent phosphofructokinase in an organism that utilizes an atypical glycolytic pathway. As such, the method described here to enable expression of ATP-dependent phosphofructokinase in an organism with an atypical glycolytic pathway will be informative toward engineering the glycolytic pathways of other industrial organism candidates with atypical glycolytic pathways.
Topics & Concepts
Clostridium thermocellumPhosphofructokinaseClostridiaceaeChemistryClostridiumThermodynamicsBiochemical engineeringBiochemistryGlycolysisBiologyBacteriaPhysicsEnzymeEngineeringGeneticsCellulaseBiofuel production and bioconversionMicrobial Metabolic Engineering and BioproductionEnzyme Structure and Function