Glutamine-Fructose-6-Phosphate Transaminase 2 (GFPT2) Is Upregulated in Breast Epithelial–Mesenchymal Transition and Responds to Oxidative Stress
Qiong Wang, Sigurður Trausti Karvelsson, Aristotelis Kotronoulas, Þórarinn Guðjónsson, Skarphéðinn Halldórsson, Óttar Rolfsson
Abstract
Epithelial-mesenchymal transition (EMT) is a cellular process inherent to cancer cell metastasis. Metabolic reprogramming is a driver of EMT. We performed proteomic profiling of three isogenic cell lines from human breast epithelium representing the epithelial, mesenchymal, and "partial" mesenchymal states of EMT to identify metabolic vulnerabilities associated with cell invasion. Bioinformatic and functional analysis revealed that the metabolic enzyme GFPT2 is a marker of claudin-low breast cancer, responds to oxidative stress, and impacts EMT, cell growth, and cell invasion.
Topics & Concepts
Epithelial–mesenchymal transitionBiologyChemistryCancer researchCell biologyDownregulation and upregulationBiochemistryGeneCancer, Hypoxia, and MetabolismFibroblast Growth Factor ResearchMetabolism, Diabetes, and Cancer