Handheld RPA-based molecular POCT system for rapid, low-cost 8-plexed detection of respiratory pathogens at home
Yunfeng Zai, Min Chao, Zunliang Wang, Yongjun Ding, Enben Su, Nongyue He
Abstract
a bidirectional flow between the lysis and RPA chambers, enhancing assay reliability. Our microfluidic design enables significant miniaturization, yielding a compact, lightweight system (<1 kg) suitable for handheld or desktop use. Its low power consumption (3 W) and remarkable cost-effectiveness ($1.4 per test) enhance the system's practicality and accessibility for home settings. Validation with 356 nasopharyngeal swabs further confirms its robust performance, achieving high sensitivity (>97%) and specificity (>99%), ensuring reliable at-home diagnosis of respiratory co-infections without requiring professional operation.
Topics & Concepts
Point-of-care testingNanotechnologyMedicineMaterials scienceImmunologySARS-CoV-2 detection and testingBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques