A multifunctional nanoprobe for targeting tumors and mitochondria with singlet oxygen generation and monitoring mitochondrion pH changes in cancer cells by ratiometric fluorescence imaging
Jingjin Zhao, Mengbing Zou, Mengjiao Huang, Liangliang Zhang, Keqin Yang, Shulin Zhao, Yi‐Ming Liu
Abstract
= 660 nm). On the other hand, in the pH range of 3-8, the fluorescence intensity ratio of BQD-FAs at wavelengths 490 nm and 650 nm showed a good linear relationship with the pH value of mitochondria. The ratiometric fluorescence imaging of mitochondria using the prepared BQD-FAs showed that when the cells were chemically stimulated with chlorphenizone, the mitochondrial pH dropped from 7.9 to 7.2 within 15 min. Based on these characteristics, we envision that the prepared multifunctional nanoprobe will be of high significance in the biomedical research of mitochondria and PDT of tumors.
Topics & Concepts
NanoprobeSinglet oxygenMitochondrionFluorescencePhotodynamic therapyCancer cellReactive oxygen speciesFluorescence-lifetime imaging microscopyChemistryBiophysicsCancerPhotochemistryOxygenBiochemistryBiologyOpticsGeneticsPhysicsOrganic chemistryAdvanced biosensing and bioanalysis techniquesNanoplatforms for cancer theranosticsCarbon and Quantum Dots Applications